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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
Current Bloomington Recipe for Drosophila Medium
start timing and cook for 10 minutes, stirring frequently. Turn off heat source. Stir in propionic acid. If you have time, cool to 70o C before adding the acid (we don't). Dispense the medium. Cool before plugging if time
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
and two drops of mannitol solution in the second 100 mm Petri dish under other dissecting microscope. Place large drop of mannitol over electrodes. 4. Place 50 - 100 embryos in one drop of M2. The number of embryos depends on the speed since the drop
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