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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311708221
中文名称：
英文名称：HB1000 HYBRIDIZATION OVEN 356 x 273 x273mm
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
11.00 ml 5.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 10% dextran sulfate 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then add to the solution at 65℃.Add hybridization
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
. 10) Wash 3x in PBTw. 11) Divide gonads among several 6 x 50 mm culture tubes (Pyrex tubes are probably best as they can be baked to kill RNase), one tube per hybridzation. Hybridization 12) Place gonads in 400 ul of 50% PBTw / 50
Analys of Genomic DNA by Southern Hybridization
far outside from samples to avoid contamination or interference with sample hybridization) 4.Submerge gel in 2000 ml 1X TAE with 0.5 µg/ml ethidium bromide. (i.e. 40 ml of 50 x TAE, 100 µl of 1% ethidium bromide stock solution, dH2 O ad 2 liter
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