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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70111-680-40
中文名称：
英文名称：2-POSITION CHILL/HEAT DRY BATH
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文献和实验Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
sterile H2 O - do not autoclave hybridization buffer; warm to 65℃ before use). Container, or bag with heat sealer, for hybridization (we have used one Kapak/Seal-a-Meal bag for as many as 40 filters on a given day). 2x SSPE (30-50 ml) diluted in water
In Situ HYBRIDIZATION TO TISSUE SECTIONS
will take a bit of practice. - 6-8 micron sections are fairly easily managed - put 1-3 sections on each subbed slide Air dry the slides for at least 20'. Fix with: 4% paraformaldehyde 20' 3X PBS 5' 1X PBS 5' 1X PBS 5' 30% EtOH
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