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文献和实验Measuring Altered Disposition of Xenobiotics in Experimental Models of Liver Disease
3‐ml syringes for femoral vein cannula PE10 polyethylene tubing: 0.6 mm × 0.28 mm Silk suture: braided silk size
microfuge tube. Freeze at -80oC or proceed directly to IP. At this point you can pre-clear the lysate by incubating with 30μl protein A/G sepharose for 30min at 4oC with rocking, then centrfuging in the cold for 1min at 14krpm and transfering the supernatant
Anderson, D. and Plewa, M.J. 1998. The international comet assay workshop. Mutagenesis. 13:67‐73. Böcker, W., Bauch, T., Müller, W.‐U., and Streffer, C. 1997
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