赛默飞世尔Thermo Fisher X50 Filters
mitex membrane plain white 142mm dia 10.0µm pore size (pack of 50) 货号：T_70311546415- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70311546415
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311546415
中文名称：
英文名称：X50 Filters mitex membrane plain white 142mm dia 10.0µm pore size (pack of 50)
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65
min in 50 µl of buffer containing 100 mM sodium phosphate (pH 7.0), 5 mM EDTA, 1 mM NADPH, 1 mM NADH, 150 µM DTT, 5 mM ß-mercaptoethanol, 20 µM acetyl-CoA or n-octanoyl-CoA or any other acyl-CoA as needed, 4% DMSO, and 8 µM of ACP pretreated with DTT
Sample preparation (analytical gels)
buffer saline,pH 7.2 to further remove cell debris and blood and then immersed into an iced PBS solution containing 3 mM EDTA,50 µg/ml leupeptine,0.2 mM PMSF and 0.8 mM benzamidine.Crypts were scraped away from the basal membrane with a scalpel.The tissue
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