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999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70311556415
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英文名称:X50 Filters mitex membrane plain white 142mm dia 5.0µm pore size (pack of 50)
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文献和实验Gel-elongation assay for type II fatty acid s
PhosphorImager scanner Procedure 1) FASII gel-elongation assay was done by preincubating 0.25 - 2 µg of E. coli or S. aureus lysate with a serial dilution of inhibitors at room temperature for 20 min in 50 µl
min in 50 µl of buffer containing 100 mM sodium phosphate (pH 7.0), 5 mM EDTA, 1 mM NADPH, 1 mM NADH, 150 µM DTT, 5 mM ß-mercaptoethanol, 20 µM acetyl-CoA or n-octanoyl-CoA or any other acyl-CoA as needed, 4% DMSO, and 8 µM of ACP pretreated with DTT
In Vitro Transcription Assay (Run-off Assay) using Permeabil
. Isolation of RNA 1. Resuspend the cell pellet in 0.5 ml of Guanidinium Thiocyanate Buffer. 2. Add 50 μl of 2 M Sodium Acetate, pH 4. Mix thoroughly by inversion. 3. Add 0.5 ml of Phenol. Mix thoroughly by inversion. 4. Add 100 μl of Chloroform:IAA. Mix
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