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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70313100703
中文名称:
英文名称:Homogeniser Stuart SHM1 handheld 220-240V 50Hz 55mm(dia) x 160mm(len) variable speed 5000 to
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
to 37°C and 65°C Sonicator Spectrophotometer Thermal cycler, automated Timer Tubes, microcentrifuge, 1.5-mL Tubes, polypropylene, 14-mL (e.g., 17- x 100-mm) for use in high-speed centrifuge Tubes, polypropylene, 50-mL
into several small screw-cap jars (~50 ml capacity) Store at room temperature 20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)
Competent cells, calcium chloride method, E. coli, description
1/100 - 1/200 to 25, 50 or 100 ml 2 X YT broth in flasks 5 - 10 X culture volume (i.e. 25 ml in 250 ml flask etc.). Grow to early log phase (OD600 = 0.2 - 0.4) (90 - 180 min depending on the strain) Collect cells by centrifugation
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