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文献和实验In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
in situs. Dissections and Fixation 1) Wearing latex gloves, prepare dissected gonads and fix in 3% paraformaldehyde / 0.25% glutaraldehyde / 0.09 M K2 HPO4 (pH7.2) (see below) for 2-3 hr at room temperature. I always do several rounds of dissections
Southern Hybridization Protocols
8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65
RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
tablet (#B5655) ; Dissolve one tablet in 10 ml staining solution. If there is a non-specific background signal, you can dilute more (up to 20 ml). Culture tubes : Available from Fisher as 6 mm (ID) x 50 mm (length
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