60105-385-B赛默飞100mg PSA, 40mg

In Situ HYBRIDIZATION TO TISSUE SECTIONS

/0.3% Triton X-100. Rinse well to remove all food and yeast. Blot well to remove excess liquid. Dechorionate embryos in freshly diluted 50% chlorox for 3' at room temperature with gentle agitation. Rinse with NaCl/Triton; blot well. Transfer

AAV最新进展

SDS)中重悬，加热40mg蛋白质酶K。37°C消化2个小时后，加入50ml 50M NaCl,冰浴4h。溶液在4°C 14000´g离心30分钟去除染色体沉淀。上清液用5ml RNase混合液(Ambion, 5mg RNase A,100单位RNaseT1)37°C处理2小时，用酚/氯仿(1:1)抽提1次，再用氯仿抽提1次。DNA用2倍体积的乙醇沉淀，用DpnI处理去除输入的质粒DNA。DNA在琼脂糖凝胶 上分离，按上述方法转移到硝酸纤维素膜上(Hirt,1967)，用Stratalinker