赛默飞世尔Thermo Fisher CPLRY 30M X

核酸电泳工作流程——5 大主要步骤

), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

or into 6 mm x 50 mm culture tubes). 5) Using a 10 mg/ml stock of Proteinase K (PrK), make solutions of 25 ug/ml and 40 ug/ml PrK in PBTw. Divide the gonads in half. Digest one half of the gonads in 3 ml of 25 ug/ml PrK / PBTw for 30 min and the other half