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文献和实验Differential Interference Contrast (Nomarski, DIC, Hoffman Modulation Contrast)
, but you may find the effect to be more dramatic. The cilia themselves can be seen if you get the paramecium to quiet down. If they won't settle right away, set the slide aside and try again later. Cilia show up best in phase contrast (400x), or in dark field at 100
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
the filter to Solution #2 and incubate for 5 minutes. 7. Transfer the filter to ddH2 O to remove excess stain. 8. Transfer the filter onto a glass slide with the migrated cells facing up; allow it to dry. 9. Use a microscope set at 200x magnification
Differential Staining of Live and Dead Embryos using Fluorescein Diacetate
for staining of all cells and not just embryos or oocytes . Also, ethididium bromide can be replaced with propidium iodide, DAPI or Hoescht 33342 Materials Ethidium Bromide (EtBr ; Fisher
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