赛默飞世尔Thermo Fisher 3M CABL PT1
00 600X6MM SS 75+250°C beschichtet, 3m Anchlusskabel 货号：T_70310214632- ￥1200 - 24000
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- 2025年07月10日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310214632
中文名称：
英文名称：3M CABL PT100 600X6MM SS 75+250°C beschichtet, 3m Anchlusskabel
货期：热销品现货，其他请咨询
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文献和实验Maxiprep of plasmid DNA from E. coli
at 13,600g/12,000rpm, 4°C, for 15 minutes. Discard the supernatant. Wash pellet with ~100μL 70% ethanol. Resuspend in 100-200μL TE buffer. Notes You may want to read this discussion of what LiCl and sodium acetate are doing in this protocol
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Purification of GST Fused Proteins
to 1 lt terrific/K2 K (see appendix) with 0.5 ml 100mg/ml Amp. Grow at 37°C to OD600 of approximately 0.5-0.8 (~90-150 min) Induce with 0.5ml 50mg/ml 1PTG prepared freshly in DDW. Incubate 2-3 hrs 37°C for stable proteins, 1 hr 37°C
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