68008-254680赛默飞250x4.6mm Hyper

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

tip) alternatively:       Finger controlled pipette (Manostat tubing, yellow tip, scalpel blade);       9'' Pasteur pipettes;       70% Ethanol;       Alcohol burner or Bunsen burner;       Sterile plastic Petri dishes (100 x 15 mm);       Sterile

Cool growth competent E . coli

2O; add 10 ml 250 mM KCl; pH to 7.0 with NaOH Adjust vol to 1 liter; Autoclave. Just before use, add 5 ml sterile 2M MgCl2 per liter TB per liter 10 mM PIPES Na salt 3.35 g 15 mM CaCl2 (Fisher) 2.2 g 250 mM KCl (Aldrich) 18.64g 55

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

(Fisher) Heating mantles (VWR) 100‐, 250‐, and 2000‐mL Erlenmeyer flasks (Kimax