赛默飞世尔Thermo Fisher Kapillarsäu

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume:       1.5 ml 1M Hepes       1.05ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       24.15ml H20 ChIP high salt buffer       30ml final volume:       1.5ml 1M Hepes    

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

into wells of a 96-well plate with 120 μl fresh media. The next day add from 0.1 to 10 μg/ml of puromycin/G418, or 2 to 32 μM ganciclovir to selected wells.       C.     Examine viability every 2 days. Replace the media containing selection

类器官培养与应用——血管类器官

%、且大部分无分化的状态时，吸出培养基。 3、向 hPSCs 中加入 0.5 mM EDTA 洗涤细胞一次。 4、吸去 EDTA 溶液，每孔加入 0.6ml Accutase，并将培养皿放置在 37℃ 解离约 3-5min，直到所有的细胞变圆。 5、向每个孔中加入 5ml 的 hPSCs 培养基，并用 0.1% 台盼蓝进行染色计数。 6、将所有细胞转移至 15ml 离心管中，室温下 300g 离心 3min，收集沉淀细胞。6 孔板中每孔接种 2×105 个细胞，加入预热的 3ml hPSCs 培养