赛默飞世尔Thermo Fisher AMMONIUM MO

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

Phosphate Assay by Suprya Jaydev

readings. 7) Add 0.3 ml of 0.5N HCl to each sample and boil for 15 minutes. 8) To each tube, add: 0.6ml ammonium molybdate 0.1 ml ascorbic acid (10% w/v, made fresh EACH time) VORTEX!! 9) Incubate for 30 minutes @ 45°C or for 60 minutes @ 37

Phosphate Assay by Suprya Jaydev

, the percipitate will appear brown/charred and this will skew the spectrophotometer readings. 7) Add 0.3 ml of 0.5N HCl to each sample and boil for 15 minutes. 8) To each tube, add: 0.6ml ammonium molybdate 0.1 ml ascorbic acid (10% w/v, made fresh