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文献和实验Beta-galactosidase Reporter Gene Assay (Liquid Form)
Beta-galactosidase Reporter Gene Assay (Liquid Form) REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
("phagemid") between the initiation site ("I") and the termination site ("T") within the central portion of the bacteriophage lambda between the two "arms" of the phage genome that are crucial for its function. Before screening, the expression library
V294.PART II，Chapter 2 Boyden法体外趋化试验
): dilute 1:10 indistilled H2O before use. 11. Peri dishes. 12. Glass slide and cover glass (32 × 24 mm). 13. Nail polish. 14. Terg-A-Zyme? (cat. no. 1304; Alconox; New York, NY): an enzyme activedetergent available from Fisher. Dissolve
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