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文献和实验a d . S ci. U SA 101, 9879-9884. 26. Bradford, M . M . (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. A n a l. B io c h e m .72, 248-254. 27.
Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
Hints and precautions for the care, feeding and breeding of Neurospora
(Perkins 1977 N.N. 24:16-17; see also N.N. 26:24-27), -20°C deep-freeze (Perkins 1973 N.N. 20:33), cryopreservation (Jong, et al. 1979 N.N. 26:26), or lyophil (Barratt et al. 1959 Science 112:122-123). The FGSC has a description of these techniques. 21
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