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文献和实验Procedures for In Vitro Production of Bovine Embryos
x g for 10 min.4. After centrifugation, collect sperm pellet from the bottom of the conical tube (Figure 16). Percoll is toxic to sperm cells and the pellet should be collected with a minimum of Percoll.Figure 16. Removal of sperm
Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs
) Triisopropylsilane (TIS, >99%, Aldrich) Diethyl ether, 4°C Acetonitrile (MeCN, HPLC‐grade, Fisher
Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
. Tris(2‐acetoxyethyl)orthoformate, pyridinium p ‐toluene‐sulfonate, 4‐tert‐butyl‐dimethyl‐siloxy‐3‐penten‐2‐one, DCM, room temperature, 48 hr, 85%; e. TEMED⋅HF, 0°C, 2 hr, quantitative; f. diisopropylamine, BzHCl, CH2 Cl2 , 0°C, 83%; g. 1 H ‐Tetrazole
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