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- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70312910161
中文名称:
英文名称:Heating mantle Bibby HM100C controllable 230V 50Hz 105W 50- 450C 100mL flask
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文献和实验Southern Hybridization Protocols
do not have the reproducibility and robustness of the former Boehringer) are used in our laboratory.Place 25ng (1 m l) of template DNA into a microcentrifuge tube. Add in 5 m l of primers and appropriate volume of 50 m l in the final reaction. Denature by heating to 95-100 ℃
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
). Preparation of host cells directly from glycerol stock Prepare a sterile 50ml conical tube, containing 10ml of LB media + 100µl of the 20% (w/v) Maltose stock solution + 100µl of the 1M MgSO4 stock solution + 10µl
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
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