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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701MP114410622
中文名称:
英文名称:SC-URA (POWDER) 30 G
货期:热销品现货,其他请咨询




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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
Large-scale yeast transformation
. Discard water and resuspend cells by vortexing in residue. We then add 140 ul of water, mix by pipetting, and plate 6 ul to SC-leu -ura plate. We use the rest of the suspension for filter assays and -70o C storage. PEG: 400 mls 50% PEG
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