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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
.) two dissecting microscopes; M2 and KSOM media; 0.3 M Mannitol (Sigma M4125) (dissolved in ultra pure water with added 0.3% BSA (Sigma A4378) and filtered through 0.22micron Millipore filter, stored in aliquots at -20 C); tissue culture dish
Peptidomic Approaches to Study Proteolytic Activity
. This unit describes the use of quantitative peptidomics in the analysis of the biological peptidome as well as in the in vitro analysis of peptidase activity. Curr. Protoc. Protein Sci. 65:18.13.1?18.13.12. © 2011 by John Wiley & Sons, Inc.
Single and Double FISH protocols for Drosophila
this was found to be unnecessary and in fact was usually detrimental. 4. Freshly prepared formaldehyde appears to be required with the high temperatures used for RNA hybridization. Commercially prepared formaldehyde solutions, even ultra-pure, generally yield ruptured embryos
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