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货号:T_70118-600
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英文名称:FUNNEL DRUM YELLOW
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文献和实验Large-scale yeast transformation
rotator, from Fisher). The boxes thus get rotated, pretty much like tubes in a roller drum. Shaking vertically in an orbital would probably be okay too. Equipment required Deepwell titer dishes: Beckman 267006 ($83/24) Capmats
Large-scale yeast transformation大规模转化酵母【Yale University】
with sterile water). Shake at 30℃ for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30℃ for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready to use. 1.We use selection because we are maintaining a plasmid. Presumably the main thing
Beta-galactosidase Reporter Gene Assay (Liquid Form)
Beta-galactosidase Reporter Gene Assay (Liquid Form) REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
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