赛默飞世尔Thermo Fisher TRAY RADIAT

基本实验技术

name, CAS#, health hazard data, including first aid treatment, physical data, fire and explosion hazard data, reactivity data, spill or leak procedures, and any special precautions needed when handling this chemical. A file containing MSDS information

Marcantonio Lab Protocol Manual

of TE buffer. Transfer to a 15 ml conical tube. Add 4.4 g of CsCl (Optical grade), dissolve by shaking, and add 0.4 ml of ethidium bromide (10 mg/ml). Caution: Ethidium bromide is a potent mutagen and an environmental hazard. Always wear gloves

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls