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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
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货号:T_70311792464
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英文名称:PROBE TYPE K DIA8X300MM PTFE
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM EDTA, pH 8.0, 2 SDS; autoclave and add 1 mM ATA and 14 mM ß-mercaptoethanol. ATA=aurintricarboxylic acid, ammonium salt (Sigma #A0885, St. Louis, MO)] 5. Thaw the powder in E buffer in 42°C
further. Wild-type embryos were washed from 60 mm plates with M9 buffer and treated for 6-10 min with a 0.5M NaOH, 5% bleach solution to release the eggs. Intact embryos were purified by flotation on 1M sucrose (Goh and Bogaert, 1991, Development 111 , 667-681
and lock box (from various vendors) Mini-centrifuge (VWR, 20668-212) Thermolyne Dri-Bath, Type 17600 (VWR, 35756-006) Platform Shaker, New Brunswick Scientific (VWR, 40000-300) Electrophoresis Separation Systems (Owl) QIAquick Nucleotide Removal Kit (Qiagen
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