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货号:T_70119-120-2403E
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英文名称:KLGRDXP LBCT COL E/W 2X 30CS
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
In Situ HYBRIDIZATION TO TISSUE SECTIONS
in xylene, 2 X 10'. Rehydrate through 100%, 95%, 80%, 60% and 30% EtOH; 1' in each solution. Incubate in 0.2N HCl, 20'. Rinse in ddH2O, 30". Incubate @ 70 degrees C in 2X SSC (preheated to 70 degrees C), 30'. Rinse in ddH
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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