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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_7012237-32
中文名称:
英文名称:COND STD, 100 5S/CM, 1 L
货期:热销品现货,其他请咨询




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文献和实验cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
[[gamma]]-[32-P]-rATP 10 uCiT4 polynucleotide kinase 1 μl (3U/ul)10 μl [[gamma]]-[32-P]-rATP (35020) ICN and T4 polynucleotide kinase (70031) from United States Biochemicals.2. Incubate at 37℃ for 30-60 minutes. 3. Heat the reaction at 65℃ for 10 minutes
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