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文献和实验原代神经细胞培养方法 Neuron Cell Culture
. Place the cells into a flask that has been coated with PDL . Add 11 ml of IMDM for a total of 13 ml in the flask B. Splitting of the astrocytes
In Situ HYBRIDIZATION TO TISSUE SECTIONS
.C.T. (Tissue-Tek O.C.T. Compound) Paraffin (Paraplast Plus, Fisher) 8% Paraformaldehyde (Polysciences E.M. Grade 0380): Prepare fresh in 1X PBS. Dissolve 8g paraformaldehyde in 100ml buffer at 65-80 degrees C. Add 1-2 drops 50% KOH
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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