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北京泽平
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货号:T_70110-200-42
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英文名称:FLASK RED SCALE 500ML 6/CS
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文献和实验Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
Large-scale yeast transformation大规模转化酵母【Yale University】
Transforming yeast Equipment required This protocol merges a typical LiAc protocol with the reducing agent component of the One-step method. It gives >103 / ug efficiency for our strain. Recipe is for 800 transformations (90 mls cells). Grow 50 ml culture
Construction and Characterization of Adenovirus Vectors
. For large-scale preparation of Ad vectors using suspension-adapted 293N3S cells: ix. Distribute a 3-L culture of 293N3S cells into eight 500-mL centrifuge bottles. x. Centrifuge at 650g for 20
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