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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311790804
中文名称：
英文名称：X6 PIPETTE 25 ML CL AS BLUE GRAD.
货期：热销品现货，其他请咨询
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
(Laemmli loading dye) 3X stock:1M Tris-Cl pH 6.8 2.4 ml20% SDS 3 mlGlycerol (100%) 3 mlB-mercaptoethanol 1.6 mlBromophenol blue 0.006g10 ml (store 4°C)10X Running buffer (also called Laemmli buffer):Tris base 30.3 gGlycine 144 gSDS 10 gmake to 1L with dH
Recombinant expression and SDS
the sample buffer in and out of the pipette tip. Note: do this SLOWLY so as not to cause the sample buffer to foam. 5. Incubate the bacteria/sample buffer mixtures at 100 °C for 5 minutes in the heating block. 6. Load 25 ml of each sample
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