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北京泽平
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货号:T_70102540J
中文名称:烧杯
英文名称:BEAKER GRIFFIN 150ML 12/PK
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文献和实验mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentr,hi) RPMI-1640 medium Tritn X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprtinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh the day f
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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