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文献和实验with sterile water). Shake at 30oC for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30oC for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3. Cells ready to use. We use selection because we are maintaining a plasmid. Presumably the main thing
600 ul beta-mercaptoethanol. Shake at 30oC for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3. Cells ready to use. We use selection because we are maintaining a plasmid. Presumably the main thing is that cells not be stationary. If you are going
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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