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文献和实验a second coverslip on top, which will flatten the agarose into a thin pad. (try to avoid air bubbles, but a few won't hurt anything) When the agarose has hardened (> 5 sec) slide off the top coverslip. Use this top coverslip as the bottom coverslip to make
with sterile water). Shake at 30oC for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30oC for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3. Cells ready to use. We use selection because we are maintaining a plasmid. Presumably the main thing
600 ul beta-mercaptoethanol. Shake at 30oC for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3. Cells ready to use. We use selection because we are maintaining a plasmid. Presumably the main thing is that cells not be stationary. If you are going
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