赛默飞世尔Thermo Fisher 玻璃体复合pH参比电极

基本实验技术

electrode. The reference electrode is often AgCl2 . An accurate pH reading depends on standardization, the degree of static charge, and the temperature of the solution. Operation of Orion PerpHecT pH Meter Expose hole on side of electrode

In Situ HYBRIDIZATION TO TISSUE SECTIONS

ml glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108

重组DNA的分离、克隆与测序实验

to 13,000 x g. for 15 minutes at 4degC. A subsequent wash with 70% ethanol, followed by brief centrifugation, removes residual salt and moisture. The general procedure for precipitating DNA and RNA is: 1. Add one-tenth volume of 3M NaOAc, pH