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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701HATF00010
中文名称:
英文名称:IMMOBILON NC TRANSFER MEMBRANE
货期:热销品现货,其他请咨询




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文献和实验Two dimensional peptide mapping
after transfer. Wrap the membrane in Saran wrap to keep the membrane wet during exposure. Try to keep the membrane damp throughout the procedure. If it dries out, you can rewet NC with water, or Immobilon with methanol and then water, but your recovery
James Hardwick CNBr Cleavage Procedure
1. Immunoprecipitate the protein and run it on a preparative gel. CNBr cleavage must be done with protein transferred to a nitrocellulose filter. Neither Immobilon nor Nylon can substitute. IMPORTANT: Wash the NC 2X for 15 min
相关专题 在电流的作用下,使蛋白质从胶转移至固相载体(膜)上。膜的选择:印迹中常用的固相材料有NC膜、DBM、DDT、尼龙膜、PVDF等。我们选用PVDF(聚偏二氟乙烯),其具有更好的蛋白吸附、物理强度,以及具有更好的化学兼容性。 有两种规格:Immobilon-P(0.45um)和Immobilon-PSQ(0.2um for MW A 半干法 即将凝胶夹层组合放在吸有转印缓冲液的滤纸之间,通过滤纸上吸附的缓冲液传导电流,起到转移的效果
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