赛默飞世尔Thermo Fisher MEMBRANE TR
ANSFER IMMOBILON ROLL IMMOBILON-PSQ 0.2µm 26.5cmx3.75m 货号：T_70310491541- ￥1200 - 24000
- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70310491541
- 2025年07月13日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310491541
中文名称：
英文名称：MEMBRANE TRANSFER IMMOBILON ROLL IMMOBILON-PSQ 0.2µm 26.5cmx3.75m
货期：热销品现货，其他请咨询
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
Recipes for the buffers are at the end of this protocol. 1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P. Neither nitrocellulose nor nylon
Phosphoamino acid analysis:Mark Kamps method
in a screw-cap tube containing 150 µl 5.7 N HCl or enough to submerge your piece of membrane. 3. Incubate in 110°C oven for 60 min. 4. Microfuge sample full speed for at least 1 min. 5. Transfer supernatant to new tube and lyophilize
James Hardwick CNBr Cleavage Procedure
samples and the membrane pieces they were released from. Your yield should be between 85-95%. 7. Resuspend in 20-40 µl SDS gel sample buffer. The sample buffer should remain blue. If it turns yellow, residual formic acid is present and will cause
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