赛默飞世尔Thermo Fisher 烧瓶 FLASK VO

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

of CTO stock solution into 18 ml of serum-free MCDB-131 medium.     4)      Rinse EPCs in 3 near confluent T-75 cell culture flasks with 10 ml DPBS (without Ca or Mg).     5)      Add 6 ml of the 2 μM CTO working solution to each flask

Construction and Characterization of Adenovirus Vectors

The total volume in the flask should be ~100 mL. xiii. Transfer the flask to the incubator. Agitate the cells at 70 rpm for 2 h. xiv. Transfer the cells to the 3-L suspension flask containing 1 L of spent

Cell Cultures

containing trypticase soy broth into the spectrophotometer and adjust the reading to 100% T. This is the blank for all subsequent measurements. 7. Transfer the remaining contents of the 12 dilutions to spectrophotometer cuvettes and measure the %T