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北京泽平
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货号:T_70315152754
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英文名称:X1 Rack Mini, 2ml, 13 cardboard boxes (25 cell divider) for CryoExtra 20, 40, 80 and 94
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
attached cells with PBS without Ca2+/Mg2+(Prod. No. D8537) using 1-2ml for each 25cm2 of surface area. Retain the washings. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover
Subculture of Suspension Cell Lines
cells in 2ml of antibiotic-free growth medium to each tissue culture dish. Incubate at 37ºC in 5% CO2 for 2 ? 24 hrs to allow the cells to adhere to the cover slips. Bring attached test cell lines into suspension using a cell scraper. Suspension cell
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