赛默飞世尔Thermo Fisher X100 VIAL F
INNERAN SHELL AMBER GLASS 1ML (PACK OF 100) 货号：T_70310370951- ￥70 - 1400
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- 库存：
999
- 供应商：
北京泽平
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- 保修期：
1年
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EA
货号：T_70310370951
中文名称：
英文名称：X100 VIAL FINNERAN SHELL AMBER GLASS 1ML (PACK OF 100)
货期：热销品现货，其他请咨询
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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
, GIBCO-BRL 11811-031) need 150ug/ml active (however, each batch should be tested. Make up X100 in PBS and filter sterilize. NB. Each batch has number of ugs/mg active written on vial, you need to allow for this when making your calculation. FIAU
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
there should be no more than approx. 100 colonies/plate. Picking of single colonies Plates are treated one at a time and as quickly as possible. Remove medium wash with PBS. Don't wash with PBS/EGTA as this causes the feeders to lift off and the ES cell colonies to float away
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