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- 库存:
999
- 供应商:
北京泽平
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货号:T_70311496358
中文名称:
英文名称:TEST TUBE RACK HALF SIZE FOR MAXQ 6000 30MM, green, 3 x 3 array
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文献和实验Procedures for In Vitro Production of Bovine Embryos
1. Prepare embryo culture medium (modified KSOM) at least 2 h before removing embryos/oocytes from the fertilization drops.2. Make enough 50 ml microdrops of culture medium (30 oocytes/embryos per drop) on 60 x 15 mm petri dishes and cover with mineral oil
In Situ HYBRIDIZATION TO TISSUE SECTIONS
hr, etc.) 2.5 ml 8% paraformaldehyde 0.5 ml 1X PBS 2.0 ml DMSO Add 5 ml heptane and shake vigorously for 20' (Buffer A) or 30' (Buffer B). Transfer to 10 ml 90% MeOH/EGTA (9ml MeOH + 1 ml 50mM EGTA
Gene Knockout Techniques(1) The Knockout Process
-stage worms with sterile M9 buffer, pellet by low-speed centrifugation in a sterile 15 ml conical centrifuge tube (2000 RPM/1 minute), and distribute with a sterile Pasteur pipette to three 150 mm RNGM plates spread with a lawn of E. coli OP
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