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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310630922
中文名称:
英文名称:X25 TLC plate Macherey-Nagel Nano-SILGUR-20 UV254 10x10 cm (VE=25Stck.)
货期:热销品现货,其他请咨询




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文献和实验dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
plate. Glycolipids are applied and TLC's developed using standard procedures. Chromatograms are then dried thoroughly (50�, 1 hr.) and allowed to cool prior to polymer coating. 2. Polyisobutylmethacrylate (see pg. 99) is prepared as a 10% (w/v
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