赛默飞世尔Thermo Fisher X2 Tube rac

超级感受态细胞的制备

. Just before use, add 5 ml of a sterile solution of 2 M MgCl2 . (This solution is made by dissolving 19 g of MgCl2 in 90 ml of deionized H2 O . Adjust the volume of the solution to 100 ml with deionized H2 O and sterilize by autoclaving for 20 minutes at 15 psi

Procedures for In Vitro Production of Bovine Embryos

OF FERTILIZED OOCYTES INTO EMBRYO CULTURE DROPS (8-10 h post IVF)Materials and Equipment Needed Vortexer Timer 1.5 ml Dolphin microcentrifuge tube and rack X-plate (with prewarmed HEPES-TALP) Plastic Pasteur pipet Heater (placed in front of the microscope) Slide

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture