赛默飞世尔Thermo Fisher MAG SLG 6.3

secondary antibody review -- data from 99 publications

ref host class subclass conjugation experiment dilution detail supplier 1 goat     Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat     Alexa Fluor 546 immunocytochemistry 1:250

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65