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- 库存:
999
- 供应商:
北京泽平
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- 保修期:
1年
- 规格:
EA
货号:T_70310194851
中文名称:
英文名称:X20 TLC PLATE CEL-300-50 UV254 fibrous cellulose MN 300 layer on glass plate for
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文献和实验Two dimensional peptide mapping
Count samples 9. Resuspend the sample in 10 microliters H2O for an 8.9 map (or appropriate electrophoresis buffer if you're using another buffer for electrophoresis ). Load 1 to 2.5¨microliters on the 0.1 mm thick, 20 cm x 20 cm cellulose
, and chromosomal DNA by pouring through a double-layer of cheesecloth into a new 50 ml conical tube. Centrifuge at 3,000 rpm for 20 minutes at 4degC in the Beckman GPR tabletop centrifuge. 6. Decant the supernatant to a 50 ml polypropylene centrifuge tube, add
Labeled Sphingomyelin Synthesis
a small level of chloroform above the packed silica. 15) Sequentially elute with the following solvents: a) 150 ml chloroform b) 150 ml acetone c) 300 ml acetone/methanol (9:1) d) 50 ml methanol e) 100 ml methanol f) 300 ml methanol g) 300 ml
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