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999
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北京泽平
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货号:T_70312987686
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英文名称:X1000 Serum separator tube BDVacutainer II advance
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
1a).Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein expressing plasmid
IntroductionA major advance in our knowledge of cells came about with the ability to maintain them in continous culture. Prokaryotes have been cultured for a relatively long time, but eukaryotic cultures were first accomplished in the early 1900's
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