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北京泽平
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货号:T_70311839143
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英文名称:LOWENSTEIN KIT 150MM TUBES
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples
【转贴】immunofluorescence staining
. Store at 4C to prolong fluorescence.Reagents and Supplies:PBS & TBS-T buffers:PBS: Standard PBS for mammalian cellsTBS-T: Tris HCl 20 mM, pH 7.4 NaCl 137mM Tween-20 0.1%PEM Buffer:Stock Solution for 1 Liter:Final Concentration: 400 mM Potassium PIPES, pH
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