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文献和实验Procedures for In Vitro Production of Bovine Embryos
in 25 ml (4-7 x 106 /ml). To do so, add 10 ml sperm suspension to 90 ml water to kill sperm. Load 10 ml of sample onto a hemacytometer. Count the number of sperm in 5 squares (Figure 18) and multiply sperm number by 500,000 to determine concentration
#______________ 1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________ X μl Nuclease free H2O to 10 μl 37℃ at least 1 hr After incubation, bring reaction volume up to 50 μl before cleaning up with spin column
Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
(see recipe ) Biological matrix: cells (grown on 25‐mm tissue culture dish) or tissue of interest Chemical agonist
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