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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70110-180H
中文名称:厚壁抽滤烧瓶4000mL
英文名称:FLASK FILTERING 4000ML
货期:热销品现货,其他请咨询




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文献和实验Neuronal Cell Dissociation Protocol
Erlenmeyer flask. Shake in orbital shaker for 10 min at 150 rpm, and transfer to a fresh 50 ml tube. 6. Meanwhile, make the culture medium (≈ 180 ml final volume): • Add 1 ml gentamycin
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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