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货号：T_70123-769-512
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
". This time interval must be constant for all samples! 10 x LUC buffer 250mM Tricine, pH 7.8 150mM MgC12. lOOmM ATP in H20 adjust to pH 7.0, sterile filter, store aliquots at -20C lOmM Luciferin stock: D(-) Luciferin lOmg (cat
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