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文献和实验Blocking cDNA arrays printed on amine slides
to do the blocking of the slides. These hold 10 slides each but we only put 6 slides in order to get the maximum blocking. Also need a small magnetic stirring bar (1/2 x 5/16 inch) and a regular stirring plate. The following amounts of reagents
Dissociated Retina Immunohistochemistry
Solutions 100X trypsin 10 mg/ml in 1X PBS Sigma #T2271 100X soybean trypsin inhibitor 10 mg/ml in 1X PBS Sigma #T6522 1000X DNaseI 2mg/ml in HBSS Sigma #D4513 Explant Culture
recommendations (Cambio). DNA FISH Culture ES cells on gelatin-coated glass coverslips or slides; Wash once in freshly made 1X PBS; Fix in filter-sterile, freshly made 3% Paraformaldehyde/1X PBS for 10 minutes at RT (see comment
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