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999
- 供应商:
北京泽平
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EA
货号:T_70310044550
中文名称:
英文名称:X12 FILTER UNITS,NALGENE,STERILE, PES MEMBRANE mm 500 ml
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文献和实验Transient transfection into 293T cells
: optional) • Transfection reagents: i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°C ii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na2 HPO4 •7H2 O, 1.0g glucose, 5.0g HEPES/500ml (adjust pH to 7.05 with NaOH and filter sterile
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Southern Transfer to Nylon Membrane in NaOH
: Day 1 Pretreatment: After staining and photographing the gel, denature by soaking in 500 ml or more of denaturing solution (0.5 N NaOH, 1.5 M NaCl) for 30 minutes with agitation. Transfer:
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